Fol. Biol. 2016, 62, 167-174

https://doi.org/10.14712/fb2016062040167

Comparison of the Radiosensitizing Effect of ATR, ATM and DNA-PK Kinase Inhibitors on Cervical Carcinoma Cells

Jiřina Vávrová1, L. Zárybnická1, P. Jošt2, A. Tichý1, M. Řezáčová3, Z. Šinkorová1, J. Pejchal1

1Department of Radiobiology, Faculty of Military Health Sciences, Hradec Králové, University of Defence Brno, Czech Republic
2Centre of Advanced Studies, Faculty of Military Health Sciences, Hradec Králové, University of Defence Brno, Czech Republic
3Institute of Medical Biochemistry, Charles University Prague, Faculty of Medicine in Hradec Králové, Czech Republic

Received November 2015
Accepted April 2016

References

1. Dai, Y., Grant, S. (2010) New insights into checkpoint kinase 1 in the DNA damage response signaling network. Clin. Cancer Res. 16, 376-383. <https://doi.org/10.1158/1078-0432.CCR-09-1029>
2. Fuhrman, C. H. B., Kilgore, J., Lacoursiere, Y. D., Lee, C.M., Milash, B. A., Soisson, A. P., Zempolich, K. A. (2008) Radiosensitization of cervical cancer cells via double-strand DNA break repair inhibition. Gynecol. Oncol. 110, 93-98. <https://doi.org/10.1016/j.ygyno.2007.08.073>
3. Fujisawa, H., Nakajima, N. I., Sunada, S., Lee, Y., Hirakawa, H., Yajima, H., Fujimori, A., Uesaka, M., Okayasu, R. (2015) VE-821, an ATR inhibitor, causes radiosensitization in human tumor cells irradiated with high LET radiation. Radiat. Oncol. 10, 175. <https://doi.org/10.1186/s13014-015-0464-y>
4. Huang, X., Tran, T., Zhang, L., Hatcher, R., Zhang, P. (2005) DNA damage-induced mitotic catastrophe is mediated by the Chk1-dependent mitotic exit DNA damage checkpoint. Proc. Natl. Acad. Sci. USA 102, 1065-1070. <https://doi.org/10.1073/pnas.0409130102>
5. Jeggo, P., Lavin, M. F. (2009) Cellular radiosensitivity: how much better do we understand it? Int. J. Radiat. Biol. 85, 1061-1081. <https://doi.org/10.3109/09553000903261263>
6. Jin, Y., Wei, Y., Xiong, L., Yang, Y., Wu, J. R. (2005) Differential regulation of surviving by p53 contributes to cell cycle dependent apoptosis. Cell Res. 15, 361-370. <https://doi.org/10.1038/sj.cr.7290303>
7. Mikusová, V., Tichý, A., Řezáčová, M., Vávrová, J. (2011) Mitoxantrone in combination with a DNA-PK inhibitor: possible therapy of promyelocytic leukaemia resistant forms. Folia Biol. (Praha) 57, 200-205.
8. Novotna, E., Tichy, A., Pejchal, J., Lukasova, E., Salovska, B., Vavrova, J. (2013) DNA-dependent protein kinase and its inhibition in support of radiotherapy. Int. J. Radiat. Biol. 89, 416-423. <https://doi.org/10.3109/09553002.2013.767993>
9. O’Drisscoll, M., Jeggo, P. A. (2003) Clinical impact of ATR checkpoint signalling failure in humans. Cell Cycle 2, 194-195. <https://doi.org/10.4161/cc.2.3.404>
10. On, K. F., Chen, Y., Ma, H. T., Chow, J. P., Poon, R. Y. (2011) Determinants of mitotic catastrophe on abrogation of the G2 DNA damage checkpoint by UCN-01. Mol. Cancer Ther. 10, 784-794. <https://doi.org/10.1158/1535-7163.MCT-10-0809>
11. Prevo, R., Fokas, E., Reaper, P. M., Charlton, P. A., Pollard, J. R., Mckenna, W. G., Muschel, R. J., Brunner, T. B. (2012) The novel ATR inhibitor VE-821 increases sensitivity of pancreatic cancer cells to radiation and chemotherapy. Cancer Biol. Ther. 13, 1072-1081. <https://doi.org/10.4161/cbt.21093>
12. Reaper, P. M., Griffiths, M. R., Long, J. M., Charrier, J. D., Maccormick, S., Charlton, P. A., Golec, J. M., Pollard, J. R. (2011) Selective killing of ATM- or p53-deficient cancer cells through inhibition of ATR. Nat. Chem. Biol. 7, 428-430. <https://doi.org/10.1038/nchembio.573>
13. Scheffner, M., Huibregtse, J. M., Vierstra, R. D., Howley, P. M. (1993) The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. Cell 75, 495-505. <https://doi.org/10.1016/0092-8674(93)90384-3>
14. Shaheen, F. S., Znojek, P., Fisher, A., Webster, M., Plummer, R., Gaughan, L., Smith, G. C., Leung, H. Y., Curtin, N. J., Robson, C. N. (2011) Targeting the DNA double strand break repair machinery in prostate cancer. PLoS One 6, e20311. <https://doi.org/10.1371/journal.pone.0020311>
15. Shang, Z. F., Huang, B., Xu, Q. Z., Zhang, S. M., Fan, R., Liu, X. D., Wang, Y., Zhou, P. K. (2010) Inactivation of DNAdependent protein kinase leads to spindle disruption and mitotic catastrophe with attenuated checkpoint protein 2 phosphorylation in response to DNA damage. Cancer Res. 70, 3657-3666. <https://doi.org/10.1158/0008-5472.CAN-09-3362>
16. Vavrova, J., Marekova, M., Vokurkova, D. (2001) Radiationinduced apoptosis and cell cycle progression in TP53-deficient human leukemia cell line HL-60. Neoplasma 48, 26-33.
17. Vavrova, J., Marekova, M., Vokurkova, D., Szkanderova, S., Psutka, J. (2003) Caffeine induces a second wave of apoptosis after low dose-rate γ radiation of HL-60 cells. Radiat. Environ. Biophys. 42, 193-199. <https://doi.org/10.1007/s00411-003-0209-4>
18. Vavrova, J., Zarybnicka, L., Lukasova, E., Rezacova, M., Novotna, E., Sinkorova, Z., Tichy, A., Pejchal, J. (2013) Inhibition of ATR kinase with the selective inhibitor VE-821 results in radiosensitization of cells of promyelocytic leukaemia (HL60). Radiat. Environ. Biophys. 52, 471-479. <https://doi.org/10.1007/s00411-013-0486-5>
19. Wagner, J. M., Kaufmann, S. H. (2010) Prospects for the use of ATR inhibitors to treat cancer. Pharmaceuticals 3, 1311-1334. <https://doi.org/10.3390/ph3051311>
20. Zhao, Y., Huw, D. T., Batey, M. A., Cowell, I. G., Richardson, C. J., Griffin, R. J., Calvert, A. H., Newell, D. R., Smith, G. C. M. (2006) Preclinical evaluation of a potent novel DNAdependent protein kinase inhibitor NU7441. Cancer Res. 66, 5354-5362. <https://doi.org/10.1158/0008-5472.CAN-05-4275>
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