Fol. Biol. 2016, 62, 203-206

https://doi.org/10.14712/fb2016062050203

Detection of Mycoplasma Contamination Directly from Culture Supernatant Using Polymerase Chain Reaction

R. V. Pisal1, H. Hrebíková1, J. Chvátalová1, D. Kunke1, S. Filip2, Jaroslav Mokrý1

1Department of Histology and Embryology, Charles University – Faculty of Medicine in Hradec Králové, Czech Republic
2Department of Oncology and Radiotherapy, Charles University – Faculty of Medicine in Hradec Králové, Czech Republic

Received February 2016
Accepted June 2016

References

1. Bolske, G. (1988) Survey of mycoplasma infections in cell cultures and a comparison of detection methods. Zentralbl. Bakteriol. Mikrobiol. Hyg. 269, 331-340.
2. Drexler, H., Uphoff, C. (2000) Contamination of cell cultures, mycoplasma. In: The Encyclopedia of Cell Technology, eds. Spier, B., Griffiths, H., Scragg, E., pp. 609-627, John Wiley & Sons, Inc., New York.
3. Kong, F., James, G., Gordon, S., Zelynski, A., Gilbert, G. (2001) Species-specific PCR for identification of common contaminant mollicutes in cell culture. Appl. Environ. Microbiol. 67, 3195-3200. <https://doi.org/10.1128/AEM.67.7.3195-3200.2001>
4. Uphoff, C., Drexler, H. (1999) Detection of mycoplasma contaminations in cell cultures by PCR analysis. Hum. Cell 12, 229-236.
5. Uphoff, C., Drexler, H. (2002) Comparative PCR analysis for detection of mycoplasma infections in continuous cell lines. In Vitro Cell. Dev. Biol. Anim. 38, 79-85. <https://doi.org/10.1290/1071-2690(2002)038<0079:CPAFDO>2.0.CO;2>
6. Uphoff, C., Denkmann, S., Drexler, H. (2012) Treatment of mycoplasma contamination in cell cultures with plasmocin. J. Biomed. Biotechnol. 2012, 267678. <https://doi.org/10.1155/2012/267678>
7. Young, L., Sung, J., Stacey, G., Masters, J. (2010) Detection of mycoplasma in cell cultures. Nat. Protoc. 5, 929-934. <https://doi.org/10.1038/nprot.2010.43>
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