The Action of Benzene, Resveratrol and Their Combination on Ovarian Cell Hormone Release

Sirotkin, Alexander V.; Kádasi, A.; Balaží, A.; Kotwica, J.; Alwasel, S.; Harrath, A. H.

doi:10.14712/fb2020066020067

Folia Biologica > Archive > 2020, Vol. 66 > Issue 2 > The Action of Benzene, Resveratrol and…

Fol. Biol. 2020, 66, 67-71

https://doi.org/10.14712/fb2020066020067

The Action of Benzene, Resveratrol and Their Combination on Ovarian Cell Hormone Release

Alexander V. Sirotkin^1,2, A. Kádasi³, A. Balaží², J. Kotwica⁴, S. Alwasel⁵, A. H. Harrath⁵

¹Constantine the Philosopher University, Nitra, Slovakia
²Department of Genetics and Reproduction, Research Institute of Animal Production, Lužianky, Slovakia
³Department of Animal Physiology, Slovak University of Agriculture in Nitra, Nitra, Slovakia
⁴Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland
⁵Kind Saud University, Department of Biology, College of Science, Riyadh, Saudi Arabia

Received November 2018
Accepted May 2020

The aim of our study was to examine the direct influence of plant polyphenol resveratrol and oil-related environmental contaminant benzene on ovarian hormone release, as well as the ability of resveratrol to prevent the effect of benzene. Porcine ovarian granulosa cells were cultured with and without resveratrol (0, 1,10 or 100 ug/ml) alone or in combination with 0.1% benzene. The release of progesterone, oxytocin and prostaglandin F was measured by enzyme immunoassay (EIA). Benzene promoted the release of progesterone, oxytocin and prostaglandin F. Resveratrol, when given alone, stimulated both progesterone and prostaglandin F, but not the oxytocin output. Moreover, resveratrol prevented and even inverted the stimulatory action of benzene on all analysed hormones. These observations demonstrate the direct influence of both benzene and resveratrol on porcine ovarian hormone release, as well as the ability of resveratrol to prevent the benzene action on the ovary.

Keywords

resveratrol, benzene, ovary, progesterone, oxytocin, prostaglandin F..

Funding

This work was supported from the Slovak Agency for Promotion of Research and Development, projects „NANOREPRO“ No. APVV-4040-11, “ENDONANOSAFE” APVV-15-0296, as well as from the Scientific Grant Agency of the Ministry of Education, Science and Sport of SR under the contracts VEGA 1/0327/16 and VEGA 1/0392/17. The authors would also like to extend their appreciation to the Researchers Supporting Project number (RSP-2020/17) at King Saud University, Riyadh, Saudi Arabia.