Changes of Hippocampal Noradrenergic Capacity in Stress Condition

Gavrilović, Ljubica; Popović, N.; Stojiljković, V.; Pejić, Snežana; Todorović, A.; Pavlović, I.; Pajović, S. B.

doi:10.14712/fb2020066020081

Folia Biologica > Archive > 2020, Vol. 66 > Issue 2 > Changes of Hippocampal Noradrenergic…

Fol. Biol. 2020, 66, 81-84

https://doi.org/10.14712/fb2020066020081

Changes of Hippocampal Noradrenergic Capacity in Stress Condition

Ljubica Gavrilović, N. Popović, V. Stojiljković, Snežana Pejić, A. Todorović, I. Pavlović, S. B. Pajović

“Vinča” Institute of Nuclear Sciences, National Institute of the Republic of Serbia, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, Serbia

Received March 2019
Accepted March 2020

This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition.

Keywords

chronic restraint stress, noradrenaline, hippocampus, rats.

Funding

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Contracts Nos. III 41027, OI 173041 and III41022.