Fol. Biol. 2021, 67, 10-15

https://doi.org/10.14712/fb2021067010010

The Effects of Different Storage Conditions and Repeated Freeze/Thaw Cycles on the Concentration, Purity and Integrity of Genomic DNA

M. Safarikova1, A. A. Kubena1, V. Frankova2, T. Zima1, Marta Kalousová1

1Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
2Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic

Received December 2020
Accepted May 2021

References

1. Cohen, J. (1992) A power primer. Psychol. Bull. 112, 155. <https://doi.org/10.1037/0033-2909.112.1.155>
2. International Organization for Standardization (2019) Molecular in vitro diagnostic examinations – Specifications for pre-examination processes for venous whole blood – Part 2: Isolated genomic DNA. ISO, 20186-2:2019.
3. Kalousová, M., Levová, K., Kuběna, A. A., Jáchymová, M., Franková, V., Zima, T. (2017) Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system). Prep. Biochem. Biotechnol. 47, 703-708. <https://doi.org/10.1080/10826068.2017.1303613>
4. Köchl, S., Niederstätter, H., Parson. W. (2005) DNA extraction and quantitation of forensic samples using the phenolchloroform method and real-time PCR. In: Forensic DNA Typing Protocols. Humana Press, Totowa, NJ.
5. Madisen, L., Hoar, D. I., Holroyd, C. D., Crisp, M., Hodes, M. E., Reynolds, J. F. (1987) The effects of storage of blood and isolated DNA on the integrity of DNA. Am. J. Med. Genet. 27, 379-390. <https://doi.org/10.1002/ajmg.1320270216>
6. Miller, S. A., Dykes, D. D., Polesky, H. F. R. N. (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16, 1215. <https://doi.org/10.1093/nar/16.3.1215>
7. Ross, K. S., Haites, N. E., Kelly, K. F. (1990) Repeated freezing and thawing of peripheral blood and DNA in suspension: effects on DNA yield and integrity. J. Med. Genet. 27, 569-570. <https://doi.org/10.1136/jmg.27.9.569>
8. Shao, W., Khin, S., Kopp, W. C. (2012) Characterization of effect of repeated freeze and thaw cycles on stability of genomic DNA using pulsed field gel electrophoresis. Biopreserv. Biobank. 10, 4-11. <https://doi.org/10.1089/bio.2011.0016>
9. Smith, S., Morin, P. A. (2005) Optimal storage conditions for highly dilute DNA samples: a role for trehalose as a preserving agent. J. Forensic Sci. 50, 1101-1108. <https://doi.org/10.1520/JFS2004411>
10. Yates, J. R., Malcolm, S., Read, A. P. (1989) Guidelines for DNA banking. Report of the Clinical Genetics Society working party on DNA banking. J. Med. Genet. 26, 245. <https://doi.org/10.1136/jmg.26.4.245>
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