Inhibition of SRSF3 Alleviates Proliferation and Migration of Gastric Cancer Cells by Regulating the PI3K/AKT/mTOR Signalling Pathway

Xie, J.; Sun, Y.; Xu, Qiaoling

doi:10.14712/fb2021067030102

Folia Biologica > Archive > 2021, Vol. 67 > Issue 3 > Inhibition of SRSF3 Alleviates…

Fol. Biol. 2021, 67, 102-107

https://doi.org/10.14712/fb2021067030102

Inhibition of SRSF3 Alleviates Proliferation and Migration of Gastric Cancer Cells by Regulating the PI3K/AKT/mTOR Signalling Pathway

J. Xie¹, Y. Sun¹, Qiaoling Xu²

¹Department of Gastroenterology, The First Affiliated Hospital of Yangtez University, the First People’s Hospital of Jingzhou, Jingzhou City, Hubei Province, China
²Department of Medical Oncology, Minhang District Oncology Hospital, Shanghai City, 200240, China

Received May 2021
Accepted August 2021

This study was aimed to investigate the impact of serine/arginine-rich splicing factor 3 (SRSF3) on the proliferation and migration of gastric cancer (GC) cells. SRSF3 levels in GC tissues and cell lines were measured by Western blotting. Functional assays were used for evaluation of GC cell proliferation, migration and invasion. The PI3K/AKT/mTOR pathway was then examined by Western blotting. SRSF3 exhibits abnormal expression for the significantly increased levels in GC. SRSF3 knockdown significantly suppressed GC progression. SRSF3 knockdown significantly inhibited activation of PI3K/AKT/mTOR signalling. Inhibition of SRSF3 alleviates proliferation and migration of GC cells, and this process is mediated by inactivation of PI3K/ AKT/mTOR signalling. Targeting SRSF3 may be a promising strategy to combat GC.