Fol. Biol. 2021, 67, 163-173
Identification of Schizosaccharomyces pombe ird Mutants Resistant to Glucose Suppression and Oxidative Stress
Glucose is both the favourite carbon and energy source and acts as a hormone that plays a regulating role in many biological processes. Calorie restriction extends the lifespan in many organisms, including Schizosaccharomyces pombe, while uptake of high glucose leads to undesired results, such as diabetes and aging. In this study, sequence analysis of Schizosaccharomyces pombe ird5 and ird11 mutants was performed using next-generation sequencing techniques and a total of 20 different mutations were detected. ird11 is resistant to oxidative stress without calorie restriction, whereas ird5 displays an adaptive response against oxidative stress. We selected nine candidate mutations located in the non-coding (6) and coding (3) region among a total of 20 different mutations. The nine candidate mutations, which are thought to be responsible for ird5 and ird11 mutant phenotypes, were investigated via forward and backward mutations by using various cloning techniques. The results of this study provide report-like information that will contribute to understanding the relationship between glucose sensing/signalling and oxidative stress response components.
Keywords
glucose suppression, oxidative stress, signalling, Schizosaccharomyces pombe, fission yeast.
Funding
This research was supported by the Istanbul University Research Fund, project No. 50428, and the Scientific and Technological Research Council of Turkey (TÜBİTAK), project No. 116Z093.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
