Tri-Lineage Differentiation of NTERA2 Clone D1 Cells towards Neural, Hepatic and Osteogenic Lineages <i>in Vitro</i>

Szeky, Balázs; Mayer, B.; Gyongy, M.; Hajdara, A.; Barsi, S.; Karpati, S.; Nemeth, K.

doi:10.14712/fb2021067050174

Folia Biologica > Archive > 2021, Vol. 67 > Issue 5-6 > Tri-Lineage Differentiation of NTERA2…

Fol. Biol. 2021, 67, 174-182

https://doi.org/10.14712/fb2021067050174

Tri-Lineage Differentiation of NTERA2 Clone D1 Cells towards Neural, Hepatic and Osteogenic Lineages in Vitro

Balázs Szeky^1,2, B. Mayer², M. Gyongy¹, A. Hajdara^1,2, S. Barsi³, S. Karpati², K. Nemeth²

¹Roska Tamás Doctoral School of Science and Technology, Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary
²Stem Cell Research Laboratory, Department of Dermatology, Venereology and Dermatooncology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
³Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Received June 2021
Accepted November 2021

Over the past decades, the in vitro use of pluripotent cell lines gained a crucial role in toxicology, preclinical drug testing and developmental biology. NTERA2 clone D1 cells were identified as pluripotent cells with high potential for neural differentiation. Although they are commonly used cellular sources in neuropharmacology and neurodevelopmental studies, their endodermal and mesodermal differentiation potential awaits further characterization. Here, we devised improved protocols for hepatogenic and osteogenic differentiation of NTERA2 clone D1 cells. Our in vitro differentiation assays showed significant up-regulation of multiple hepatogenic markers. We also observed robust mineralization and osteogenic marker expression of NTERA2 clone D1 cells upon in vitro osteogenic induction. These results suggest that NTERA2 clone D1 cells may be utilized as an in vitro model system to study various aspects of liver biology and osteogenesis. In addition, tri-lineage differentiation of NTERA2 clone D1 cells may serve as a simple experimental control system when validating pluripotency of other cell types.

Erratum to this article was published in: Folia Biologica, 2023, 69, (1): 40–40. https://doi.org/10.14712/fb2023069010040

Keywords

NTERA2 clone D1 cells, neurogenesis, hepatogenesis, osteogenesis, tri-lineage differentiation.

Funding

This work was supported by the Hungarian National Research, Development and Innovation Office (Grant NN 114460 to SK). The research has been supported by the ÚNKP-19-3 New National Excellence Programme of the Ministry of Human Capacities, Hungary (Grant ÚNKP-19-3-III-PPKE-30). The research project has been partially supported by the European Union, cofinanced by the European Social Fund (EFOP-3.6.3-VEKOP-16-2017-00002).