Fol. Biol. 2022, 68, 45-49
https://doi.org/10.14712/fb2022068020045
Time and Temperature Stability of TGF-β1, EGF and IGF-1 in 20% and 100% Human Serum
Katerina Jirsova1, K. Levova2, Marta Kalousová2, I. Fales3, V. Frankova4, V. Vesela1, T. Zima2, T. P. Utheim5, Jan Bednár1
1Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
2Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
3Department of Cell Therapy and Cord Blood Bank Czech Republic, Institute of Haematology and Blood Transfusion, Prague, Czech Republic
4Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
5Department of Medical Biochemistry, Oslo University Hospital and Department of Plastic and Reconstructive Surgery, Oslo University Hospital, Norway
Received January 2022
Accepted February 2022
References
1. Benito, M. J., Calder, V., Corrales, R. M., Garcia-Vazquez, C., Narayanan, S., Herreras, J. M., Stern, M. E., Calonge, M., Enriquez-de-Salamanca, A. (2013) Effect of TGF-beta on ocular surface epithelial cells. Exp. Eye Res. 107, 88-100.
<https://doi.org/10.1016/j.exer.2012.11.017>
2. Bradley, J. C., Bradley, R. H., McCartney, D. L., Mannis, M. J. (2008) Serum growth factor analysis in dry eye syndrome. Clin. Exp. Ophthalmol. 36, 717-720.
<https://doi.org/10.1111/j.1442-9071.2008.01895.x>
3. Bradley, J. C., Simoni, J., Bradley, R. H., McCartney, D. L., Brown, S. M. (2009) Time- and temperature-dependent stability of growth factor peptides in human autologous serum eye drops. Cornea 28, 200-205.
<https://doi.org/10.1097/ICO.0b013e318186321e>
4. Espinosa, A., Hjorth-Hansen, H., Aasly, K., Teigum, I., Sivertsen, G., Seghatchian, J. (2015) Implementation of a standardised method for the production of allogeneic serum eye drops from regular blood donors in a Norwegian University Hospital: some methodological aspects and clinical considerations. Transfus. Apher. Sci. 53, 88-91.
<https://doi.org/10.1016/j.transci.2015.05.014>
5. Fischer, K. R., Opitz, A., Boeck, M., Geerling, G. (2012) Stability of serum eye drops after storage of 6 months. Cornea 31, 1313-1318.
<https://doi.org/10.1097/ICO.0b013e3182542085>
6. Geerling, G., Maclennan, S., Hartwig, D. (2004) Autologous serum eye drops for ocular surface disorders. Br. J. Ophthalmol. 88, 1467-1474.
<https://doi.org/10.1136/bjo.2004.044347>
7. Giannaccare, G., Versura, P., Buzzi, M., Primavera, L., Pellegrini, M., Campos, E. C. (2017) Blood derived eye drops for the treatment of cornea and ocular surface diseases. Transfus. Apher. Sci. 56, 595-604.
<https://doi.org/10.1016/j.transci.2017.07.023>
8. , M. B., Khaw, P. T., Schultz, G. S., Adams, J. L., Shimizu, R. W. (1992) Effects of epidermal growth factor, fibroblast growth factor, and transforming growth factorbeta on corneal cell chemotaxis. Invest. Ophthalmol. Vis. Sci. 33, 3292-3301.
9. Jirsova, K., Juklova, K., Vesela, V., Filipec, M. (2006) Morphological and immunocytochemical characterization of snake-like chromatin cells. Histol. Histopathol. 21, 355- 360. Jirsova, K., Brejchova, K., Krabcova, I., Filipec, M., Al Fakih, A., Palos, M., Vesela, V. (2014) The application of autologous serum eye drops in severe dry eye patients; subjective and objective parameters before and after treatment. Curr. Eye Res. 39, 21-30.
<https://doi.org/10.3109/02713683.2013.824987>
10. Jirsova, K., Seidler Stangova, P., Palos, M., Mahelkova, G., Kalasova, S., Rybickova, I., Utheim, T. P., Vesela, V. (2020) Aberrant HLA-DR expression in the conjunctival epithelium after autologous serum treatment in patients with graftversus- host disease or Sjogren's syndrome. PLoS One 15, e0231473.
<https://doi.org/10.1371/journal.pone.0231473>
11. Klenkler, B., Sheardown, H., Jones, L. (2007) Growth factors in the tear film: role in tissue maintenance, wound healing, and ocular pathology. Ocul. Surf. 5, 228-239.
<https://doi.org/10.1016/S1542-0124(12)70613-4>
12. Kojima, T., Ishida, R., Dogru, M., Goto, E., Matsumoto, Y., Kaido, M., Tsubota, K. (2005) The effect of autologous serum eyedrops in the treatment of severe dry eye disease: a prospective randomized case-control study. Am. J. Ophthalmol. 139, 242-246.
<https://doi.org/10.1016/j.ajo.2004.08.040>
13. Lambiase, A., Micera, A., Pellegrini, G., Merlo, D., Rama, P., De Luca, M., Bonini, S., Bonini, S. (2009) In vitro evidence of nerve growth factor effects on human conjunctival epithelial cell differentiation and mucin gene expression. Invest. Ophthalmol. Vis. Sci. 50, 4622-4630.
<https://doi.org/10.1167/iovs.08-2716>
14. Lopez-Garcia, J. S., Garcia-Lozano, I., Rivas, L., Ramirez, N., Raposo, R., Mendez, M. T. (2014) Autologous serum eye drops diluted with sodium hyaluronate: clinical and experimental comparative study. Acta Ophthalmol. 92, e22- e29.
<https://doi.org/10.1111/aos.12167>
15. , J. S., Garcia-Lozano, I., Rivas, L., Ramirez, N., Mendez, M. T., Raposo, R. (2016) Stability of growth factors in autologous serum eyedrops after long-term storage. Curr. Eye Res. 41, 292-298.
16. Matsumoto, Y., Dogru, M., Goto, E., Ohashi, Y., Kojima, T., Ishida, R., Tsubota, K. (2004) Autologous serum application in the treatment of neurotrophic keratopathy. Ophthalmology 111, 1115-1120.
<https://doi.org/10.1016/j.ophtha.2003.10.019>
17. Murube, J., Rivas, L. (2003) Impression cytology on conjunctiva and cornea in dry eye patients establishes a correlation between squamous metaplasia and dry eye clinical severity. Eur. J. Ophthalmol. 13, 115-127.
<https://doi.org/10.1177/112067210301300201>
18. Na, K. S., Kim, M. S. (2012) Allogeneic serum eye drops for the treatment of dry eye patients with chronic graft-versushost disease. J. Ocul. Pharmacol. Ther. 28, 479-483.
<https://doi.org/10.1089/jop.2012.0002>
19. Nishida, T., Ohashi, Y., Awata, T., Manabe, R. (1983) Fibronectin. A new therapy for corneal trophic ulcer. Arch. Ophthalmol. 101, 1046-1048.
<https://doi.org/10.1001/archopht.1983.01040020048007>
20. Phasukkijwatana, N., Lertrit, P., Liammongkolkul, S., Prabhasawat, P. (2011) Stability of epitheliotrophic factors in autologous serum eye drops from chronic Stevens-Johnson syndrome dry eye compared to non-autoimmune dry eye. Curr. Eye Res. 36, 775-781.
<https://doi.org/10.3109/02713683.2011.587935>
21. Ripa, M., Jabbehdari, S., Yazdanpanah, G., Lukacs, E., Karcher, B., Iqbal, O., Bouchard, C. (2020) The role of multisystem disease in composition of autologous serum tears and ocular surface symptom improvement. Ocul. Surf. 18, 499- 504.
<https://doi.org/10.1016/j.jtos.2020.02.011>
22. RStudio Team (2020) RStudio: Integrated Development Environment for R. RStudio, PBC, Boston. Retrieved from http://www.rstudio.com/
23. Tsubota, K., Goto, E., Fujita, H., Ono, M., Inoue, H., Saito, I., Shimmura, S. (1999a) Treatment of dry eye by autologous serum application in Sjogren's syndrome. Br. J. Ophthalmol. 83, 390-395.
<https://doi.org/10.1136/bjo.83.4.390>
24. Tsubota, K., Goto, E., Shimmura, S., Shimazaki, J. (1999b) Treatment of persistent corneal epithelial defect by autologous serum application. Ophthalmology 106, 1984-1989.
<https://doi.org/10.1016/S0161-6420(99)90412-8>
25. Wirostko, B., Rafii, M., Sullivan, D. A., Morelli, J., Ding, J. (2015) Novel therapy to treat corneal epithelial defects: a hypothesis with growth hormone. Ocul. Surf. 13, 204-212 e201.
<https://doi.org/10.1016/j.jtos.2014.12.005>
26. Yoon, K. C., Heo, H., Im, S. K., You, I. C., Kim, Y. H., Park, Y. G. (2007) Comparison of autologous serum and umbilical cord serum eye drops for dry eye syndrome. Am. J. Ophthalmol. 144, 86-92.
<https://doi.org/10.1016/j.ajo.2007.03.016>
