Fol. Biol. 2022, 68, 201-205
https://doi.org/10.14712/fb2022068050201
Technical Notes No Inhibitory Effect of Heparinized Blood on Real-Time PCR Analysis of Thrombophilic Mutations
References
1. , P., Staji, H., Sani, R. N. (2022) Comparison of three methods including temperature, H2O2/ascorbic acid/sonication, and nitrous acid treatments for overcoming the inhibitory effect of heparin on DNA amplification in realtime-PCR. Int. J. Biol. Macromol. 209, 1298-1306.
<https://doi.org/10.1016/j.ijbiomac.2022.04.113>
2. , X., Fischer, S., Keshavjee, S., Liu, M. (2000) Heparin interference with reverse transcriptase polymerase chain reaction of RNA extracted from lungs after ischemia-reperfusion. Transpl. Int. 13, 146-150.
<https://doi.org/10.1111/j.1432-2277.2000.tb01055.x>
3. , M., Koblížek, V., Dulíček, P., Hegerová, J., Palička, V. (2022) Whole blood samples for faster real-time PCR analysis of thrombophilic mutations in SARS-CoV-2 virus positive patients. Physiol. Res. 71, 439-445.
<https://doi.org/10.33549/physiolres.934883>
4. , E., Gelbart, T., Kuhl, W. (1990) Interference of heparin with the polymerase chain reaction. Biotechniques 9, 166.
5. , D., Behrmann, O., Hufert, F., Dame, G., Urban, G. (2018) Direct DNA and RNA detection from large volumes of whole human blood. Sci. Rep. 8, 3410.
<https://doi.org/10.1038/s41598-018-21224-0>
6. , M., Bullotta, A., Caruso, L., Gupta, P., Rinaldo, C. R., Chen, Y. (2011) An optimized sensitive method for quantitation of DNA/RNA viruses in heparinized and cryopreserved plasma. J. Virol. Methods 176, 1-8.
<https://doi.org/10.1016/j.jviromet.2011.05.012>
7. , M. E., Blanco, J. L., Caballero, J., Gargallo-Viola, D. (2002) Anticoagulants interfere with PCR used to diagnose invasive aspergillosis. J. Clin. Microbiol. 40, 1567-1568.
<https://doi.org/10.1128/JCM.40.4.1567-1568.2002>
8. , S., Proper, J. A., Bowie, E. J., Sommer, S. S. (1987) Parameters affecting the yield of DNA from human blood. Anal. Biochem. 165, 294-299.
<https://doi.org/10.1016/0003-2697(87)90272-7>
9. , M., Kim, S., Katzenstein, D., Konrad, M., Groves, E., Merigan, T. C. (1991) Inhibition of human immunodeficiency virus gene amplification by heparin. J. Clin. Microbiol. 29, 676-679.
<https://doi.org/10.1128/jcm.29.4.676-679.1991>
10. , Y., Meguro, S., Kanno, K., Hiraki, H., Nemoto, U., Hata, R., Takahashi, K., Miura, Y., Yoshida, H. (2002) The effect of EDTA contaminated in sera on laboratory data. Clin. Chim. Acta 325, 105-111.
<https://doi.org/10.1016/S0009-8981(02)00251-6>
11. , S., Pfleiderer, C., Lion, T. (1991) Detection of gene expression by PCR amplification of RNA derived from frozen heparinized whole blood. Nucleic Acids Res. 19, 6051.
<https://doi.org/10.1093/nar/19.21.6051>
12. , K. L., Sabatini, L. M., Tsongalis, G. J., Caliendo, A. M., Olsen, R. J., Ashwood, E. R., Bale, S., Benirschke, R., Carlow, D., Funke, B. H., Grody, W. W., Hayden, R. T., Hegde, M., Lyon, E., Murata, K., Pessin, M., Press, R. D., Thomson, R. B. (2017) The case for laboratory developed procedures: quality and positive impact on patient care. Acad. Pathol. 4, 2374289517708309.
<https://doi.org/10.1177/2374289517708309>
13. , A. S., Bartley, A. N., Bridge, J. A., Kamel-Reid, S., Lazar, A. J., Lindeman, N. I., Long, T. A., Merker, J. D., Rai, A. J., Rimm, D. L., Rothberg, P. G., Vasalos, P., Moncur, J. T. (2018) Comparison of laboratory-developed tests and FDA-approved assays for BRAF, EGFR, and KRAS testing. JAMA Oncol. 4, 838-841.
<https://doi.org/10.1001/jamaoncol.2017.4021>
14. , K., Kurapeev, D., Popov, M., Sidorova, M., Minasian, S., Galagudza, M., Kostareva, A., Fedorov, A. (2016) Heparinase treatment of heparin-contaminated plasma from coronary artery bypass grafting patients enables reliable quantification of microRNAs. Biomol. Detect. Quantif. 8, 9-14.
<https://doi.org/10.1016/j.bdq.2016.03.001>
15. , S., Chen, H., Song, J., Lee, C., Geng, Q. (2016) Avoiding heparin inhibition in circulating MicroRNAs amplification. Int. J. Cardiol. 207, 92-93.
<https://doi.org/10.1016/j.ijcard.2016.01.129>
16. , M. (2018) Harmonization in laboratory medicine: more than clinical chemistry? Clin. Chem. Lab. Med. 56, 1579-1586.
<https://doi.org/10.1515/cclm-2017-0865>
17. M., Sciacovelli, L., Aita, A., Chiozza, M. L. (2014) Harmonization of pre-analytical quality indicators. Biochem. Med. (Zagreb) 24, 105-113.
<https://doi.org/10.11613/BM.2014.012>
18. , H. P., Ijzermans, J. N. M., van der Laan, L. J. W. (2021) Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids. BMC Biotechnol. 21, 48.
<https://doi.org/10.1186/s12896-021-00706-6>
19. , E., Ali, D., Dumontet, E., Le Carrer, D., Orsonneau, J. L., Delaroche, O., Bigot-Corbel, E. (2014) Influence of anticoagulant on the plasma level of fifteen biochemical parameters. Ann. Biol. Clin. (Paris) 72, 337-350.
20. , J. T., Wang, T. H., Sheu, J. C., Lin, S. M., Lin, J. T, Chen, D. S. (1992) Effects of anticoagulants and storage of blood samples on efficacy of the polymerase chain reaction assay for hepatitis C virus. J. Clin. Microbiol. 30, 750-753.
<https://doi.org/10.1128/jcm.30.3.750-753.1992>
21. , M., Tatsumi, N., Nathalang, O., Yamada, T., Tsuda, I. (1999) Effects of heparin on polymerase chain reaction for blood white cells. J. Clin. Lab. Anal. 13, 133-140.
<https://doi.org/10.1002/(SICI)1098-2825(1999)13:3<133::AID-JCLA8>3.0.CO;2-0>
