Fol. Biol. 2023, 69, 69-73

https://doi.org/10.14712/fb2023069020069

Regulation of Dihydropyrimidinase-like 3 Gene Expression by MicroRNAs in PC12 Cells with Induced Ischaemia and Hypothermia

Kisang Kwon1, Ji-Hye Song2, Hyewon Park3, O-Yu Kwon3, Seung-Whan Kim4

1Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan, South Korea
2Institute of Bioscience and Integrative Medicine, College of Korean Medicine, Daejeon University, Daejeon, South Korea
3Department of Anatomy and Cell Biology, College of Medicine, Chungnam National University, Daejeon, South Korea
4Department of Emergency Medicine, College of Medicine, Chungnam National University, Daejeon, South Korea

Received December 2022
Accepted September 2023

Although hypothermic treatment has been reported to have some beneficial effects on ischaemia at the clinical level, the mechanism of ischaemia suppression by hypothermia remains unclear due to a lack of mechanism understanding and insufficient data. The aim of this study was to isolate and characterize microRNAs specifically expressed in ischaemia-hypothermia for the dihydropyrimidinase-like 3 (Dpysl3) gene. PC12 cells were induced with CoCl2 for chemical ischaemia and incubated at 32 ℃ for hypothermia. In ischaemia-hypothermia, four types of microRNAs (miR-106b-5p, miR-194-5p, miR-326-5p, and miR-497-5p) were highly related to the Dpysl3 gene based on exosomal microRNA analysis. Dpysl3 gene expression was up-regulated by miR-497-5p but down-regulated by miR-106b-5p, miR-194-5p and miR-326-5p. Our results suggest that these four microRNAs are involved in the regulation of Dpysl3 gene expression. These findings provide valuable clues that exosomal microRNAs could be used as therapeutic targets for effective treatment of ischaemia.

Funding

This study (NRF-2020R1I1A3074228) was supported by Korean National Research Foundation (NRF) and by Chungnam National University Hospital Research Fund, 2013.

References

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