Fol. Biol. 2025, 71, 18-28
Clinical Value and Regulatory Mechanism of miR-767-5p in Colorectal Cancer
, Xiuju Qin2, Caiyun Yi3, Man Jiang4, Lili Yi3, Yuemian Liang5
1Department of General Surgery, Minimally Invasive Surgery Center, Second People’s Hospital of Hunan Province (Hunan Brain Hospital), Changsha, China
2Department of Oncology, No. 971 Navy Hospital of the Chinese People’s Liberation Army, Qingdao, China
3Department of Nursing, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, China
4Department of Hospice, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, China
5Department of Pathology, Affiliated Hospital of Hebei University, Baoding, China
Received November 2024
Accepted February 2025
The poor prognosis of colorectal cancer (CRC) contributes to a yearly increase in CRC mortality, while microRNAs (miRNAs) were found to play a regulatory function in diversiform cancers, including CRC. The objective of this research was to evaluate the clinical value and possible regulatory mechanisms of miR-767-5p in CRC. The expression level of miR-767-5p in CRC tissues and cells was examined. The Kaplan-Meier curve was utilized to analyse the function of miR-767-5p in CRC prognosis. The independent prognostic factors in CRC were assessed by a multivariate COX regression analysis. Additionally, the regulatory mechanism of miR-767-5p in CRC was determined through an in vitro cell experiment. The miR-767-5p expression was down-regulated in CRC tumour tissues and CRC cells. Indicators such as tumour differentiation, TNM, LNM and miR-767-5p were identified as independent prognostic factors for a poor CRC prognosis. The regulatory relationship between miR-767-5p and nuclear factor I A (NFIA) was verified by the dual-luciferase reporter assay, and the NFIA expression level was significantly suppressed by over-expressed miR-767-5p. The proliferation, migration and invasion of CRC cells were inhibited by over-expressing miR-767-5p, while the inhibition effect could be reversed by over-expressing NFIA. The over-expressed miR-767-5p could serve as a tumour suppressor to inhibit the progression of CRC by suppressing the expression level of NFIA.
Supplementary materials: Supplementary Table 1 (pdf file)
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