Fol. Biol. 2008, 54, 134-140
Determination of Angiogenic Factors in Serum by Protein Array in Patients with Renal Cell Carcinoma
Using the protein array method we determined the serum levels of a number of angiogenic factors. We identified serum levels of angiogenin, PDGF and MCP-1 (CCL2 chemokine) in serum of 32 patients with RCC, and 14 healthy volunteers by means of antibody array analysis. The patients were divided into three groups according to their disease stages (I+II, III, and IV). We found significant differences between the controls and patients with RCC both pre-operatively and post-operatively in angiogenin, PDGF and MCP-1 serum levels. The increase in angiogenin, PDGF and MCP-1 lasted in patients with RCC stages I-III even without metastases eight weeks post-operatively. The patients with stage IV RCC showed disturbed production of PDGF and MCP-1. Protein array analysis is a powerful tool for the identification of large numbers of trace proteins. Multiplex antibody array is able to provide data more precisely reflecting the nature of pathological processes.
Keywords
renal cell carcinoma, angiogenin, PDGF, MCP-1, protein array method.
Funding
This study was supported by grant from the Internal Grant Agency of the Ministry of Health of the Czech Republic NR/8914-4.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
