Fol. Biol. 2011, 57, 26-29
HVRII of mtDNA in Cord Blood Cells of Newborn Children and in Their Saliva 10 Years Later
Comparison of hypervariable region II nucleotide sequences of mitochondrial DNA obtained from cord blood cells and saliva cells of the same individual at birth and after ten years revealed a few differences at the so-called mutation hot spots (three transitions and three indels within the C-tract). The personal identity of samples was proved by short tandem repeat profiling. Comparison of individuals living in two regions that differ by air pollution, however, did not reveal statistically significantly increased number of mutations in the population from the region of poorer environmental conditions, although indicating such tendency.
Keywords
variability of mtDNA, hypervariable region II, comparison of cord blood cells and saliva cells from ten years old children, comparison of two regions.
Funding
The present report is based on sample collection performed during the period 1994–2007, which was supported by the Ministry of Environment of the Czech Republic VaV/SL/160/05.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
