Association between a Marker on Chromosome 9 and Acute Coronary Syndrome Confirmatory Study on Czech Population

Hubáček, Jaroslav A.; Staněk, V.; Gebauerová, M.; Poledne, R.; Aschermann, M.; Skalická, H.; Matoušková, J.; Kruger, A.; Pěnička, M.; Hrabáková, H.; Veselka, J.; Hájek, P.; Lánská, V.; Adámková, V.; Piťha, J.

doi:10.14712/fb2012058050203

Folia Biologica > Archive > 2012, Vol. 58 > Issue 5 > Association between a Marker on…

Fol. Biol. 2012, 58, 203-208

https://doi.org/10.14712/fb2012058050203

Association between a Marker on Chromosome 9 and Acute Coronary Syndrome Confirmatory Study on Czech Population

Jaroslav A. Hubáček¹, V. Staněk¹, M. Gebauerová¹, R. Poledne¹, M. Aschermann², H. Skalická², J. Matoušková³, A. Kruger³, M. Pěnička⁴, H. Hrabáková⁴, J. Veselka⁵, P. Hájek⁵, V. Lánská¹, V. Adámková^1,6, J. Piťha¹

¹Institute for Clinical and Experimental Medicine, Prague, Czech Republic
²2nd Department of Internal Medicine, General University Hospital, Prague, Czech Republic
³Department of Cardiology, Homolka Hospital, Prague, Czech Republic
⁴Cardiocenter, Department of Cardiology, University Hospital Královské Vinohrady, Prague, Czech Republic
⁵Department of Cardiology, Teaching Hospital Motol, Prague, Czech Republic
⁶University of South Bohemia, Faculty of Health and Social Studies, České Budějovice, Czech Republic

Received February 2012
Accepted March 2012

Myocardial infarction (MI) is the leading cause of death in industrialized countries. All the classical risk factors for MI are responsible for approximately 50 % of MI cases. Attention has therefore recently been attracted to those genetic variants that are not associated with conventional risk factors. One of them is the marker rs10757274 in the “genefree” zone on chromosome 9, which has been repeatedly recognized as a risk factor for development of MI in Western populations. We analysed the relationship between the rs10757274 variant on chromosome 9 and risk of the acute coronary syndrome (ACS) in Czech population. The rs10757274 (A > G) variant was successfully analysed (CR = 99.4 % for patients and 98.4 % for controls) by PCR-RFLP in consecutively examined 1,046 men and 281 women with ACS (age below 65 years) and in population-based controls – 1,162 men and 1,355 women (aged up to 65 years). ANOVA and χ² were used for statistical analysis. We confirmed that GG homozygotes are more frequent (codominant model of analysis) among patients with myocardial infarction than in the control group both inmen(28.5%vs.22.0%,P=0.0001,OR1.73,95% CI 1.36–2.19) and women (32.0 % vs. 24.6 %, P = 0.02, OR 1.62, 95 % CI 1.13–2.34). However, rs10757274 polymorphism was not associated with the classical risk factors either in control population or in ACS patients. We conclude that the rs10757274 variant at 9p23.1 is an important genetic risk factor for ACS development in the Czech population.

Keywords

acute coronary syndrome, ANRIL, polymorphism, chromosome 9, CDKN2A, CDKN2B.

Funding

Supported by the project of Ministry of Health of the Czech Republic for development of research organization 00023001 (IKEM, Prague, Czech Republic) – Institutional support and project NT 12217-5 (IGA. MH. CR).