Fol. Biol. 2014, 60, 10-20

https://doi.org/10.14712/fb2014060010010

Efficient ELISA for Diagnosis of Active Tuberculosis Employing a Cocktail of Secretory Proteins of Mycobacterium tuberculosis

D. Tiwari1,2,3, R. P. Tiwari4, R. Chandra2, P. S. Bisen5, Shafiul Haque6

1Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa
2Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi, India
3School of Chemistry & Physics, University of KwaZulu-Natal, Durban, South Africa
4Department of Biotechnology, Immunodiagnostic Division, Aspen Laboratories Pvt. Ltd., Delhi, India
5School of Studies in Biotechnology, Jiwaji University, Gwalior, MP, India
6Department of Biosciences, Jamia Millia Islamia (A Central University), New Delhi, India

Received July 2013
Accepted September 2013

References

1. Abebe, F., Holm-Hansen, C., Wiker, H. G., Bjune, G. (2007) Progress in serodiagnosis of Mycobacterium tuberculosis infection. Scand. J. Immunol. 66, 176-191. <https://doi.org/10.1111/j.1365-3083.2007.01978.x>
2. Al-Zarouni, M., Dash, N., Al Ali, M., Al-Shehhi, F., Panigrahi, D. (2010) Tuberculosis and MDR-TB in the northern Emirates of United Arab Emirates: a 5-year study. Southeast Asian J. Trop. Med. Public Health 41, 163-168.
3. Andersen, P., Askgaard, D., Ljungqvist, L., Bennedsen, J., Heron, I. (1991) Proteins released from Mycobacterium tuberculosis during growth. Infect. Immun. 59, 1905-1910. <https://doi.org/10.1128/iai.59.6.1905-1910.1991>
4. Anderson, P. (1994) Effective vaccination of mice against Mycobacterium tuberculosis infection with a soluble mixture of secreted Mycobacterial proteins. Infect. Immun. 62, 2536-2544. <https://doi.org/10.1128/iai.62.6.2536-2544.1994>
5. Beyene, D., Franken, K. L., Yamuah, L., Aseffa, A., Wiker, H. G., Kolk, A., Engers, H., Klatser, P., Sviland, L. (2010) Serodiagnosis of tuberculous lymphadenitis using a combination of antigens. J. Infect. Dev. Ctries. 4, 96-102. <https://doi.org/10.3855/jidc.116>
6. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. <https://doi.org/10.1016/0003-2697(76)90527-3>
7. Daleine, G. (1995) Preliminary evaluation of a Mycobacterium tuberculosis lipooligosaccharide (LOS) antigen in the serogical diagnosis of tuberculosis in HIV seropositive and seronegative patients. Tuber. Lung Dis. 76, 234-239. <https://doi.org/10.1016/S0962-8479(05)80011-5>
8. Daniel, T. M., Debanne, S. M. (1987) The serodiagnosis of tuberculosis and other mycobacterial diseases by enzymelinked immunosorbent assay. Am. Rev. Respir. Dis. 135, 1137-1151.
9. Espitia, C., Cervera, I., Gonzalez, R., Mancilla, R. (1989) A 38 kD Mycobacterium tuberculosis antigen associated with infection. Its isolation and serological evaluation. Clin. Exp. Immunol. 77, 373-377.
10. Fine, P. E. (1995) Variation in protection by BCG: implications of and for heterologous immunity. Lancet 346, 1339-1345. <https://doi.org/10.1016/S0140-6736(95)92348-9>
11. Garberi, J., Labrador, J., Garberi, F., Garberi, J. E., Peneipil, J., Garberi, M., Scigliano, L., Troncoso, A. (2011) Diagnosis of Mycobacterium tuberculosis using molecular biology technology. Asian Pac. J. Trop. Biomed. 1, 89-93. <https://doi.org/10.1016/S2221-1691(11)60002-6>
12. Garg, S. K., Tiwari, R. P., Tiwari, D., Singh, R., Malhotra, D., Ramnani, V. K., Prasad, G. B. K. S., Chandra, R., Fraziano, M., Colizzi, V., Bisen, P. S. (2003) Diagnosis of tuberculosis: available technologies, limitations and possibilities. J. Clin. Lab. Anal. 16, 1-7.
13. Grange, J. M. (1998a) The genus Mycobacterium. In: Mycobacteria and Human Disease, pp. 9-30, Edward Arnold Ltd., London.
14. Grange, J. M. (1998b) The species of Mycobacterium. In: Mycobacteria and Human Disease, pp. 31-48, Edward Arnold Ltd., London.
15. Grange, J. M., Laszlo, A. (1990) Serodiagnostic test for tuberculosis: a need for assessment of their operational predictive accuracy and acceptability. WHO Bull. O.M.S. 68, 571-576.
16. Griffin, J. F., Nagai, S., Buchan, G. S. (1991) Tuberculosis in domesticated red deer: comparison of purified protein derivative and the specific protein MPB70 for in vitro diagnosis. Res. Vet. Sci. 50, 279-285. <https://doi.org/10.1016/0034-5288(91)90124-7>
17. Gupta, S., Shende, N., Kumar, S., Harinath, B. C. (2005) Detection of antibodies to a cocktail of mycobacterial excretory-secretory antigens in tuberculosis by ELISA and immunoblotting. Curr. Sci. 88, 1825-1827.
18. Harinath, B. C., Kumar, S., Roy, S. S., Hirudkar, S., Upadhye, V., Shende, N. (2006) A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis. Diagn. Microbiol. Infect. Dis. 55, 65-68. <https://doi.org/10.1016/j.diagmicrobio.2005.10.021>
19. Jones, B. E., Young, S. M., Antoniskis, D., Davidson, P. T., Kramer, F., Barnes, P. F. (1998) Relationship of the manifestations of tuberculosis to CD4 cell counts in patients with human immunodeficiency virus infection. Am. Rev. Respir. Dis. 148, 1292-1297. <https://doi.org/10.1164/ajrccm/148.5.1292>
20. Kamath, A. T., Feng, C. G., Macdonald, M., Briscoe, H., Britton, W. J. (1999) Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis. Infect. Immun. 67, 1702-1707. <https://doi.org/10.1128/IAI.67.4.1702-1707.1999>
21. Kanaujia, G. V., Motzel, S., Garcia, M. A., Andersen, P., Gennaro, M. L. (2004) Recognition of ESAT-6 sequences by antibodies in sera of tuberculous nonhuman primates. Clin. Diagn. Lab. Immunol. 11, 222-226.
22. Kumar, G., Dagur, P. K., Singh, P. K., Shankar, H., Yadav, V. S., Katoch, V. M., Bajaj, B., Gupta, R., Sengupta, U., Joshi, B. (2010) Serodiagnostic efficacy of Mycobacterium tuberculosis 30/32 kDa Mycolyl transferase complex, ESAT-6, and CFP-10 in patients with active tuberculosis. Arch. Immunol. Ther. Exp. 58, 57-65. <https://doi.org/10.1007/s00005-009-0055-4>
23. Laemmli, U. K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-985. <https://doi.org/10.1038/227680a0>
24. Nagai, S., Wiker, H. G., Harboe, M., Kinomoto, M. (1991) Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect. Immun. 59, 372-382. <https://doi.org/10.1128/iai.59.1.372-382.1991>
25. Pratt, R. P., Roser, B. (1989) Comparison of blocking agents for ELISA. Thermo Scientific Nunc Bulletin 7, 1-3.
26. Rebeski, D. E., Winger, E. M., Shin, Y. K., Robinson, M. L. M. M., Varecka, R., Crowther, J. R. (1999) Identification of unacceptable background caused by non-specific protein adsorption to the plastic surface of 96-well immunoassay plates using a standardized enzyme-linked immunosorbent assay procedure. J. Immunol. Methods 226, 85-92. <https://doi.org/10.1016/S0022-1759(99)00051-4>
27. Sani, M., Houben, E. N., Geurtsen, J., Pierson, J., de Punder, K., van Zon, M., Wever, B., Piersma, S. R., Jiménez, C. R., Daffé, M., Appelmelk, B. J., Bitter, W., van der Wel, N., Peters, P. J. (2010) Direct visualization by cryo-EM of the mycobacterial capsular layer: a labile structure containing ESX-1-secreted proteins. PLoS Pathog. 6, 1-10. <https://doi.org/10.1371/journal.ppat.1000794>
28. Shin, A. R., Shin, S. J., Lee, K. S., Eom, S. H., Lee, S. S., Lee, B. S., Lee, J. S., Cho, S. N., Kim, H. J. (2008) Improved sensitivity of diagnosis of tuberculosis in patients in Korea via a cocktail enzyme-linked immunosorbent assay containing the abundantly expressed antigens of the K Strain of Mycobacterium tuberculosis. Clin. Vaccine Immunol. 15, 1788-1795. <https://doi.org/10.1128/CVI.00231-08>
29. Spencer, J. S., Kim, H. J., Marques, A. M., Gonzalez-Juarerro M., Lima, M. C., Vissa, V. D., Truman, R. W., Gennaro, M. L., Cho, S. N., Cole, S. T., Brennan P. J. (2004) Comparative analysis of B and T cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10. Infect. Immun. 72, 3161-3170. <https://doi.org/10.1128/IAI.72.6.3161-3170.2004>
30. Suhail, A. (2011) Pathogenesis, immunology, and diagnosis of latent mycobacterium tuberculosis infection. Clin. Dev. Immunol. 17, 2011.
31. Tiwari, R. P., Tiwari, D., Garg, S. K., Chandra, R., Bisen, P. S. (2005) Glycolipids of M. tuberculosis strain H37Rv are potential serological markers for diagnosis of active tuberculosis. J. Clin. Diagn. Lab. Immunol. 12, 465-473.
32. Tiwari, R. P., Garg, S. K., Bharmal, R. N., Kartikeyan, S., Bisen, P. S. (2007a) Rapid liposomal agglutination card for the detection of antigens in patients with active tuberculosis. Int. J. Tuberc. Lung Dis. 11, 1143-1151.
33. Tiwari, R. P., Hattikudur, N. S., Bharmal, R. N., Kartikeyan, S., Deshmukh, N. M., Bisen, P. S. (2007b) Modern approaches to a rapid diagnosis of tuberculosis: promises and challenges ahead. Tuberculosis 87, 193-201. <https://doi.org/10.1016/j.tube.2006.07.005>
34. Toman, K. (1981) Sensitivity, specificity and predictive value of diagnostic tests. Bull. Int. Tuberculosis 56, 19-30.
35. Vogt, R. F. Jr., Phillips, D. L., Henderson, L. O., Whitfield, W., Spierto, F. W. (1987) Quantitative differences among various proteins as blocking agents for ELISA microtiter plates. J. Immunol. Methods 101, 43-50. <https://doi.org/10.1016/0022-1759(87)90214-6>
36. Volkman, H. E., Pozos, T. C., Zheng, J., Davis, J. M., Rawls, J. F., Ramakrishnan, L. (2010) Tuberculous granuloma induction via interaction of a bacterial secreted protein with host epithelium. Science 327, 466-469. <https://doi.org/10.1126/science.1179663>
37. Weldingh, K., Rosenkrands, I., Jacobsen, S., Rasmussen, P. B., Elhay, M. J., Andersen, P. (1998) Two-dimensional electrophoresis for analysis of Mycobacterium tuberculosis culture filtrate and purification and characterization of six novel proteins. Infect. Immun. 66, 3492-3500. <https://doi.org/10.1128/IAI.66.8.3492-3500.1998>
38. World Health Organization. Global Tuberculosis Report 2012.
39. Wu, X., Yang, Y., Zhang, J., Li, B., Liang, Y., Zhang, C., Dong, M., Cheng, H., He, J. (2010) Humoral immune responses against the Mycobacterium tuberculosis 38-kilodalton, MTB48, and CFP-10/ESAT-6 antigens in tuberculosis. Clin. Vaccine Immunol. 17, 372-375. <https://doi.org/10.1128/CVI.00287-09>
40. Young, Y. B., Suk, A. K., Ji-Soo, K., Hyung-Jin, E., Bai, G. H., Cho, S. N., Yu, S. K. (2004) Antigens secreted from Mycobacterium tuberculosis: identification by proteomics approach and test for diagnostic marker. Proteomics 4, 3299-3307.
front cover

ISSN 0015-5500 (Print) ISSN 2533-7602 (Online)

Open access journal

Archive