Genetic Variants in Haem Oxygenase-1 and Endothelial Nitric Oxide Synthase Influence the Extent and Evolution of Coronary Artery Atherosclerosis

Král, Aleš; Kovárník, T.; Králík, L.; Skalická, H.; Horák, J.; Mintz, G. S.; Uhrová, J.; Sonka, M.; Wahle, A.; Downe, R.; Aschermann, M.; Martásek, P.; Linhart, A.

doi:10.14712/fb2011057050182

Folia Biologica > Archive > 2011, Vol. 57 > Issue 5 > Genetic Variants in Haem Oxygenase-1 and…

Fol. Biol. 2011, 57, 182-190

https://doi.org/10.14712/fb2011057050182

Genetic Variants in Haem Oxygenase-1 and Endothelial Nitric Oxide Synthase Influence the Extent and Evolution of Coronary Artery Atherosclerosis

Aleš Král¹, T. Kovárník¹, L. Králík², H. Skalická¹, J. Horák¹, G. S. Mintz³, J. Uhrová⁴, M. Sonka⁵, A. Wahle⁵, R. Downe⁵, M. Aschermann¹, P. Martásek², A. Linhart¹

¹Second Department of Medicine – Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic
²Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic
³Cardiovascular Research Foundation, New York, NY, USA
⁴Institute of Clinical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic
⁵Department of Electrical and Computer Engineering, The University of Iowa, Iowa City, IA, USA

Received June 2011
Accepted June 2011

The genetic basis for atherosclerosis development and progression is poorly characterized. We aimed to assess the relationship between endothelial nitric oxide synthase (ENOS) 894 G/T, haem oxygenase-1 (HO1) dinucleotide-length promoter polymorphisms and coronary artery atherosclerotic involvement and its changes during statin therapy. Coronary angiography, intravascular ultrasound (IVUS), IVUS-derived virtual histology (VH) and genetic polymorphism analysis were performed at study entry. Patients were randomized 1 : 1 to standard or aggressive hypolipidaemic treatment, and a follow-up evaluation was performed after twelve months. Plaque magnitude was significantly higher in carriers of HO1 risk variants when compared with carriers of the protective variants (< 25 GT repeats). Similarly, the total coronary atherosclerotic burden was significantly greater in HO1 risk variant carriers than in HO1 protective variant carriers. Both parameters did not differ with respect to the ENOS genotype. A higher prevalence of thin-cap fibroatheroma (TCFA) in HO1 risk variant carriers was observed, compared with the HO1 protective variant carriers. The prevalence of TCFA was not influenced by the ENOS genotype. Baseline plaque composition did not differ significantly with respect to both polymorphisms. Significant interactions between plaque composition changes and ENOS and HO1 genotypes were observed during statin treatment. In conclusion, the protective HO1 promoter polymorphism correlates with a lower coronary artery plaque burden, whereas the protective ENOS 894 G/T polymorphism seems to favourably influence changes of coronary artery plaque composition during statin therapy, but has no significant correlation to the magnitude of coronary atherosclerosis.